Nephroprotective and Antioxidant Potential of Ethanolic Extract of Flowers of Cassіa Sіamea against Gentamicin Induced Nephrotoxicity

Abstract

To investigate the nephroprotective and antioxidant activity of Ethanolic extract of Cassіa Sіamea (EECS) against Gentamicin (100 mg/kg, p.o. for 7 days) induced kidney damage in rats. Rats were pretreated with EECS (250 and 500 mg/kg, p.o) 30 min prior to Gentamicin ingestion for seven days. The extent of defense was measured using levels of serum enzymes like creatinine, uric acid and blood urea nitrogen (BUN). Additionally, oxidative stress parameters such as levels of Malondialdehyde (MDA), reduced glutathione (GSH) and activity of superoxide dismutase (SOD) and catalase (CAT) along with histological evaluation of kidney sections was carried out to shore up the induction of kidney scratch and nephroprotective potential. The substantially elevated kidney weight and serum enzyme levels of creatinine, uric acid and BUN. Oxidative stress parameters MDA, GSH levels and SOD, CAT activities were found to be restored towards normalization by EECS comparable with silymarin standard. Pathological changes were in same road supports finding of biochemical evidences of nephroprotection. The total phenolic content and the total flavonoid content of the extract were 21.55 ± 2.54 mg catechol equivalent/g and 24.50 ± 2.00 mg quercetin equivalent/g respectively. EECS possess an extremely hopeful antioxidant and nephroprotective potential against Gentamicin induced kidney damage.